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| Brand | MultiSciences |
| CatNum | 70-EK310 |
| Product Name | Rat IL-10 ELISA Kit |
| Customs Name | Rat IL-10 ELISA Kit |
| Application | ELISA |
| Reactivity | Rat |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4 (unopened) standard stored at -20, others stored at 4 (opened) |
| Shipping Condition | 4 |
| Sample Volume | 20 l |
| Sensitivity | 0.48 pg/ml |
| Standard Curve Range | 62.50 - 4000 pg/ml |
| Spike Recovery Range | 86 % - 108 % |
| Mean Spike Recovery | 0.98 |
| CV of Intra plate | 3.4 % - 5.3 % |
| CV of Inter plate | 4.5 % - 6.1 % |
| Release Date | 2016/8/20 |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against IL-10 Rat IL-10 Standard, lyophilized IL-10 Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10) Substrate (TMB) Stop Solution Washing Buffer (20) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat IL-10. The Rat IL-10 ELISA is for research use only. Not for use in therapeutic procedures. Rat Interleukin 10 (IL-10) cDNA encodes a 178 amino acid (aa) residue precursor protein that contains an 18 aa signal sequence and a 160 aa mature protein. Mature rat IL-10 shares 85% and 74% aa sequence identity with mouse and human IL-10, respectively. IL-10 is primarily produced by monocytes and, to a lesser extent, lymphocytes, namely type 2 T helper cells (Th2), mastocytes, CD4+ CD25+ Foxp3+ regulatory T cells, and in a certain subset of activated T cells and B cells. IL-10 is a cytokine with multiple, pleiotropic, effects in immunoregulation and inflammation. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-B activity, and is involved in the regulation of the JAK-STAT signaling pathway. The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations. IL-10 can furthermore exert strong anti-inflammatory activities. |
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